The thesis deals with the increasing of business efficiency of the online store 4RIDERS. The aim of the thesis is the complex analysis, evaluation and recommendation of specific steps to modernize the online store 4RIDERS in terms of online marketing activities, strategies and technical solutions. The theoretical part describes the legislative and technical requirements necessary for the operation of the e-commerce and defines the basic concepts of online marketing and its tools, including online marketing strategy See-Think-Do-Care. In the practical part is processed by the situational analysis, analysis and evaluation of the current online marketing strategy according to the tools display ads, SEO, PPC, social networking, email marketing ...