Conformational transitions in a 4-way DNA junction when titrated with ionic solutions are studied using time-resolved fluorescence resonance energy transfer. Parameters characterising the transition in terms of critical ion concentration (c 1/2) and the Hill coefficient for ion binding are obtained by fitting a simple two-state model using steady-state spectra. Data obtained from a fluorescence lifetime plate reader and analysed by fitting a single exponential to donor fluorescence lifetime decays are shown to be in good agreement with the parameters obtained from steady-state measurements. Fluorescence lifetimes, however, offer advantages, particularly in being independent of fluorophore concentration, output intensity, inhomogeneity in th...
During the last fifteen years several methods have been developed for probing biomolecules (DNA, RNA...
Inter- or intramolecular distances of biomolecules can be studied by Forster resonance energy transf...
Switchable FRET is the combination of single-molecule Förster resonance energy transfer (smFRET) wit...
Conformational transitions in a 4-way DNA junction when titrated with ionic solutions are studied us...
The four-way DNA (Holliday) junction is an important postulated intermediate in the process of genet...
Time-resolved fluorescence resonance energy transfer has been used to investigate the conformational...
This article demonstrates a new fluorescence resonance energy transfer (FRET) based approach to dete...
AbstractFluorescence resonance energy transfer (FRET) measured at the single-molecule level can reve...
Förster resonance energy transfer (FRET) is a powerful method for obtaining information about small-...
Abstract Förster resonance energy transfer (FRET) is a powerful method for obtaining information ab...
The analysis of structure and dynamics of biomolecules is important for understanding their function...
We describe a method for distinguishing between minor groove binders and base intercalators that is ...
Holliday Junctions are critical DNA intermediates central to double strand break repair and homologo...
The analysis of structure and dynamics of biomolecules is important for understanding their function...
Interactions between proteins can be demonstrated by fluorescence resonance energy transfer (FRET) [...
During the last fifteen years several methods have been developed for probing biomolecules (DNA, RNA...
Inter- or intramolecular distances of biomolecules can be studied by Forster resonance energy transf...
Switchable FRET is the combination of single-molecule Förster resonance energy transfer (smFRET) wit...
Conformational transitions in a 4-way DNA junction when titrated with ionic solutions are studied us...
The four-way DNA (Holliday) junction is an important postulated intermediate in the process of genet...
Time-resolved fluorescence resonance energy transfer has been used to investigate the conformational...
This article demonstrates a new fluorescence resonance energy transfer (FRET) based approach to dete...
AbstractFluorescence resonance energy transfer (FRET) measured at the single-molecule level can reve...
Förster resonance energy transfer (FRET) is a powerful method for obtaining information about small-...
Abstract Förster resonance energy transfer (FRET) is a powerful method for obtaining information ab...
The analysis of structure and dynamics of biomolecules is important for understanding their function...
We describe a method for distinguishing between minor groove binders and base intercalators that is ...
Holliday Junctions are critical DNA intermediates central to double strand break repair and homologo...
The analysis of structure and dynamics of biomolecules is important for understanding their function...
Interactions between proteins can be demonstrated by fluorescence resonance energy transfer (FRET) [...
During the last fifteen years several methods have been developed for probing biomolecules (DNA, RNA...
Inter- or intramolecular distances of biomolecules can be studied by Forster resonance energy transf...
Switchable FRET is the combination of single-molecule Förster resonance energy transfer (smFRET) wit...