Effects of two representative antitumor ruthenium(III) complexes on thermal denaturation profiles of DNA
- Messori, L
- Casini, Angela
- Vullo, D
- Haroutiunian, S.G
- Dalian, E.B
- Orioli, P
DOIAbstract
The effects of two representative antitumor ruthenium(III) complexes — namely (dichloro-1,2-propylenediamine tetraacetate ruthenium(III)) Ru(III)) RAP and (sodium trans-(dimethylsulfoxide)(imidazole)tetrachloro ruthenate(III)) NAMI — on thermal denaturation profiles of calf thymus DNA were analyzed. It is shown that both complexes slightly stabilize calf thymus DNA when tested in the concentration range 0.01<r<0.1; minor effects on the melting interval ΔT are also observed. Differential melting curves permit monitoring of the effects of ruthenium complexes, on the different regions of calf thymus DNA depending on their GC content. Preferential binding of NAMI to GC-rich tracts of DNA is observed. Notably both complexes favor renaturation of...
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