Developing a fast, inexpensive, and specific test that reflects the mutations present in Mycobacterium tuberculosis isolates according to geographic region is the main challenge for drug-resistant tuberculosis (TB) control. The objective of this study was to develop a molecular platform to make a rapid diagnosis of multidrug-resistant (MDR) and extensively drug-resistant TB based on single nucleotide polymorphism (SNP) mutations present in therpoB, katG, inhA,ahpC, and gyrA genes from Colombian M. tuberculosis isolates. The amplification and sequencing of each target gene was performed. Capture oligonucleotides, which were tested before being used with isolates to assess the performance, were designed for wild type and mutated codons, and t...
ABSTRACTObjectiveTo design a rapid test to detect the rifampin (RIF) and isoniazid (INH) resistant m...
Progress in understanding the basis of resistance to isoniazid (INH) and rifampin (RMP) has allowed ...
The frequency of individual genetic mutations conferring drug resistance (DR) to Mycobacterium tuber...
Multi- drug resistant tuberculosis continues to be a serious problem, particularly among some develo...
Past genotypic studies of Mycobacterium tuberculosis may have incorrectly estimated the importance o...
AbstractMultidrug-resistant tuberculosis (MDRTB) is a serious world health problem that limits publi...
Molecular diagnostic methods based on the detection of mutations conferring drug resistance are prom...
Reliable molecular diagnostics, which detect specific mutations associated with drug resistance, are...
Previous evaluations of the molecular GenoType tests have promoted their use to detect resistance to...
AbstractObjectiveTo evaluate multiplex allele specific polymerase chain reaction as a rapid molecula...
During the early development of drug resistance in Mycobacterium tuberculosis (M. tuberculosis) infe...
In Mycobacterium tuberculosis (Mtb) the detection of single nucleotide polymorphisms (SNPs) is of hi...
In Mycobacterium tuberculosis (Mtb) the detection of single nucleotide polymorphisms (SNPs) is of hi...
Objective: To design a rapid test to detect the rifampin (RIF) and isoniazid (INH) resistant mutant ...
Introduction: In this study, some molecular methods were designed for rapid detection of resistance ...
ABSTRACTObjectiveTo design a rapid test to detect the rifampin (RIF) and isoniazid (INH) resistant m...
Progress in understanding the basis of resistance to isoniazid (INH) and rifampin (RMP) has allowed ...
The frequency of individual genetic mutations conferring drug resistance (DR) to Mycobacterium tuber...
Multi- drug resistant tuberculosis continues to be a serious problem, particularly among some develo...
Past genotypic studies of Mycobacterium tuberculosis may have incorrectly estimated the importance o...
AbstractMultidrug-resistant tuberculosis (MDRTB) is a serious world health problem that limits publi...
Molecular diagnostic methods based on the detection of mutations conferring drug resistance are prom...
Reliable molecular diagnostics, which detect specific mutations associated with drug resistance, are...
Previous evaluations of the molecular GenoType tests have promoted their use to detect resistance to...
AbstractObjectiveTo evaluate multiplex allele specific polymerase chain reaction as a rapid molecula...
During the early development of drug resistance in Mycobacterium tuberculosis (M. tuberculosis) infe...
In Mycobacterium tuberculosis (Mtb) the detection of single nucleotide polymorphisms (SNPs) is of hi...
In Mycobacterium tuberculosis (Mtb) the detection of single nucleotide polymorphisms (SNPs) is of hi...
Objective: To design a rapid test to detect the rifampin (RIF) and isoniazid (INH) resistant mutant ...
Introduction: In this study, some molecular methods were designed for rapid detection of resistance ...
ABSTRACTObjectiveTo design a rapid test to detect the rifampin (RIF) and isoniazid (INH) resistant m...
Progress in understanding the basis of resistance to isoniazid (INH) and rifampin (RMP) has allowed ...
The frequency of individual genetic mutations conferring drug resistance (DR) to Mycobacterium tuber...