Repeated sprint cycling is an effective training method in promoting athletic performance of cyclists, which may induce severe fatigue of lower limb muscles. However, the relationship between the fatigue of each lower limb muscles and the changing of exercise performance remains unclear. In this study, ten cyclist volunteers performed a series of 6-second sprints with 24-s recovery for five times. Power, cadence, and EMG mean frequency (MNF) of each lower limb muscle group for every 2-second epoch, as well as the grey relational grade between exercise performance and MNF of each lower limb muscle group during the whole process were calculated. It has been found that MNF of Rectus femoris (RF), Vastus (VAS), Gastrocnemius (GAS), and the hams...