The aim of this study was to denote biochemical soil activity in pure Scots pine, Norway spruce, silver fir, European larch, European beech and oak stands as well as in mixed fir-pine, beech-pine and fir-beech forests growing on a fertile fresh mixed deciduous site. The field work was carried out in the following Forest Districts: Nowe Ramuki (Mazursko-Podlaska forest region), Płońsk, Jabłonna, Brzeziny Siedlce, Grójec (Mazowiecko-Podlaska forest region) and Skarżysko, Ostrowiec and Marcule (Małopolska forest region). In 2015–2017, sample plots were assigned and chemical as well as soil enzyme activity measurements were made in each forest stand. Samples were taken from the organic (O) and humus (A) layers and for both the acidity (in 1M KC...