Isolation and Molecular Characterization of Rummelii Bacillus Stabekisii: An Efficient Protease Producing Bacterial Strain Identified from Environmental Waste Samples of Warangal District in Telangana

The exploration of novel sources of commercially important enzymes is important due to the enormous industrial requirement. Modern isolation, screening and characterization techniques expedited such efforts. Protease is a highly specific and one of the most important primary enzymes having extensive industrial applications. It is being used in almost all the industrial sectors for one or the other hydrolyzing requirement.The aim of the present study was to isolate, screen, and performing molecular characterization of protease producing novel bacterial strain, sampled from the environmental waste soil around the Warangal region. A total of 25 samples were collected and serial dilution was done with 0.1ml of the sample, further,spreading was done on nutrient agar medium and the sample was incubated at 37⁰C for 24 to 48 hrs.Proteolytic activity in the colony-forming microorganisms was detected through skimmed milk agar medium. The results suggest that samples collected from the tannery waste soil showed high protease activity. A total of 10 colonies of TWSS-P-2 showed high protease activity through larger hydrolysis zone creation (33.5mm).Morphological and biochemical characters were studied based on their isolate and molecular characterization confirmed the strains as Rummelli Bacillus Stabekisii (TWSS-P-2).The maximum activityof the selected strains was observed at pH 7.2 and at temperature 37⁰C.We have conducted several production enhancement and optimization experiments using different carbon and nitrogen sources. Among different carbon and nitrogen sources studied, the highest protease activity of 596(U/ml) was found optimal. Our analysis confirmed that the most effective bacterial strain for the sampled soil was Rummelli Bacillus Stabekisii (WSS-P-2)