DETERMINATION OF WATER-SOLUBLE VITAMINS USING HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY

This paper considers the possibility to comprehensive quantification of pyridoxine hydrochloride, thiamine hydrochloride, cyanocobalamin, lidocaine hydrochloride, benzyl alcohol are selected of “Vitagamma solution for intramuscular injection in ampoules of 2 ml per pack №5×2” drug by HPLC method. The expediency of using 0.1 M sodium orthophosphate solution with pH level equal to 5 as a buffer solution and introduction of 0.001 M sodium pentansulfonat as an ion pair agent is proved. The conditions for chromatographic determination of active ingredients in the drug are selected: a gradient of concentration of the organic solvent (acetonitrile) from 2 to 60%; chromatographic column ZORBAX Eclipse Plus C8, 3.0×150 mm, 3.5-Micron; column temperature 30 °C; mobile phase velocity of 0.6 ml/min; spectrophotometric detector wavelength of 245 nm; sample volume of 20 ul