MODIFICATION AND METHOD OF DETERMINING OF ACTIVE GROUPS OF HEPARIN ON THE SURFACE OF SUTURE MATERIAL

The coating with the application of biopolymers of polyhydroxyalkanoate class and unfractionated heparin is perspective for giving thromboresistant properties to a surgical suture. We used a chemical reaction which was performed in several stages to give strength and uniformity to the antithrombotic coating. The first stage was the initiation of the centers of graft copolymerization on the surface of the material by ozonation. At the second stage we created an additional underlayer of polymethacryloylchloride having active acid chloride groups in its content, which can form strong covalent links with heparin. The third stage was a chemical grafting of heparin on the formed underlayer from its solution in bicarbonate buffer at a reduced temperature. Quality assessment of heparin addition to the suture material was evaluated by Raman scattering spectroscopy. The obtained results showed that the modification of the suture material with the help chemical initiation method allows to firmly secure the heparin on the surface of the suture