Bone Marrow-Derived Mesenchymal Stem Cells Differentially Affect Glioblastoma Cell Proliferation, Migration, and Invasion: A 2D-DIGE Proteomic Analysis

Shenjie Li
Wei Xiang
Junjie Tian
Haorun Wang
Shuiwang Hu
Ke Wang
Ligang Chen
Changren Huang
Jie Zhou

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Publication date

January 2021

DOI

10.1155/2021/4952876

Publisher

Hindawi Limited

Journal

BioMed Research International

Abstract

Bone marrow-derived mesenchymal stem cells (BM-MSCs) display high tumor tropism and cause indirect effects through the cytokines they secrete. However, the effects of BM-MSCs on the biological behaviors of glioblastoma multiforme remain unclear. In this study, the conditioned medium from BM-MSCs significantly inhibited the proliferation of C6 cells (P<0.05) but promoted their migration and invasion (P<0.05). Two-dimensional fluorescence difference gel electrophoresis (2D-DIGE) proteomic analysis revealed 17 proteins differentially expressed in C6 cells exposed to the BM-MSC-conditioned medium including five upregulated proteins and 12 downregulated proteins. Among these, six differentially expressed proteins (Calr, Set, Oat, Npm1, Ddah1, an...