Identification and expression analysis of MYB transcription factor family in spinach

In this study,76 transcription factor genes within MYB gene family were identified. According to their structural characteristics, these MYB genes were divided into two categories, i.e.R2R3-MYB and R1R2R3-MYB.Based on the Prot Param, Soft Berry,MEGA7.0 and other online softwares were applied for bioinformatics analyses,such as physical and chemical properties,subcellular localization,and system evolution. According to their MYB conserved domain numbers,76 spinach (Spinacia oleracea) MYB genes were identified and divided into two subgroups,2R-MYB (72) and R1R2R3-MYB(4). Chromosome mapping shows, spinach's MYB family has 32 genes in the chromosome chain, the other 44 genes are located in the reverse chain of chromosomes.Conservative domain analysis shows that, the conserved domains in the MYB DNA binding domain are mainly located between the second and third spirals of the two R repeats while the amino acid sequences between the first and second tryptophan repeats of each R in the DNA binding domain are relatively unconservative. The phylogenetic analysis indicated that spinach, Arabidopsis thaliana and beet MYB transcription factor proteins can be divided into thirty-five clusters.It was speculated that the 56 members,which can be functionally divided into 4 groups,of the spinach MYB family,plays an important regulatory role in the growth and development of spinach.The seven MYB genes from other members may be involved in the process of nitrogen utilization and development in response to nitrogen concentration in spinach