Analysis of protein-DNA interactions in chromatin by UV induced cross-linking and mass spectrometry
- Alexandra Stützer
- Luisa M. Welp
- Monika Raabe
- Timo Sachsenberg
- Christin Kappert
- Alexander Wulf
- Andy M. Lau
- Stefan-Sebastian David
- Aleksandar Chernev
- Katharina Kramer
- Argyris Politis
- Oliver Kohlbacher
- Wolfgang Fischle
- Henning Urlaub
Publication date
October 2020
DOI Publisher
Springer Science and Business Media LLC Journal
Nature CommunicationsAbstract
Cross-linking mass spectrometry (XLMS) allows mapping of protein-protein and protein-RNA interactions, but the analysis of protein-DNA complexes remains challenging. Here, the authors develop a UV light-based XLMS workflow to determine protein-DNA interfaces in reconstituted chromatin and isolated nuclei
Add to ORKG