In the presence of porcine pancreatic elastase, Tb3+ ions can be excited by energy transfer from an aromatic side chain of the protein. The enhancement of Tb3+ luminescence (λex = 280 nm; λem = 543 nm) due to binding of Tb3+ to elastase is ∼2 × 104. The luminescence excitation spectrum indicates that energy transfer is from a tryptophan side chain to the bound Tb3+. Analysis of the luminescence intensity data shows that Tb3+ ions bind to elastase, forming a 1:1 complex, with an association constant of 3 × 105 M-1 (pH 6.58, 25 °C). Binding constants for Ca2+, Mg2+, and Zn2+ to elastase were also determined by the luminescence method (displacement of Tb3+), showing that under physiological conditions elastase exists as Ca2+·elastase. Addition...
Enzymes play critical roles in the regulation of cellular function and are implicated in numerous di...
Type II restriction enzymes are homodimeric systems that bind four to eight base pair palindromic re...
AbstractThe synthetic substrate anthraniloyl-β-glycerol-P binds to myoinositol monophosphatase with ...
The binding of Tb3+ and other lanthanides to Con A has been studied by sensitized Tb3+ luminescence,...
The binding of Tb3+ and other lanthanides to Con A has been studied by sensitized Tb3+ luminescence,...
The binding of Tb3+ and other lanthanides to Con A has been studied by sensitized Tb3+ luminescence,...
The luminescent isomorphous Ca2+ analogue, Tb3+, can be bound in the 12-amino acid metal binding sit...
Author Institution: Department of Chemistry, University of VirginiaInteractions of calcium ions with...
Author Institution: Department of Chemistry, University of VirginiaInteractions of calcium ions with...
Cycopiazonic acid (CPA) is a neurotoxin that acts through inhibition of the sarco(endo)plasmic retic...
It is well known that the activity of calcineurin (CaN) could be modulated by several transitional m...
Cycopiazonic acid (CPA) is a neurotoxin that acts through inhibition of the sarco(endo)plasmic retic...
Enzymes play critical roles in the regulation of cellular function and are implicated in numerous di...
Enzymes play critical roles in the regulation of cellular function and are implicated in numerous di...
Enzymes play critical roles in the regulation of cellular function and are implicated in numerous di...
Enzymes play critical roles in the regulation of cellular function and are implicated in numerous di...
Type II restriction enzymes are homodimeric systems that bind four to eight base pair palindromic re...
AbstractThe synthetic substrate anthraniloyl-β-glycerol-P binds to myoinositol monophosphatase with ...
The binding of Tb3+ and other lanthanides to Con A has been studied by sensitized Tb3+ luminescence,...
The binding of Tb3+ and other lanthanides to Con A has been studied by sensitized Tb3+ luminescence,...
The binding of Tb3+ and other lanthanides to Con A has been studied by sensitized Tb3+ luminescence,...
The luminescent isomorphous Ca2+ analogue, Tb3+, can be bound in the 12-amino acid metal binding sit...
Author Institution: Department of Chemistry, University of VirginiaInteractions of calcium ions with...
Author Institution: Department of Chemistry, University of VirginiaInteractions of calcium ions with...
Cycopiazonic acid (CPA) is a neurotoxin that acts through inhibition of the sarco(endo)plasmic retic...
It is well known that the activity of calcineurin (CaN) could be modulated by several transitional m...
Cycopiazonic acid (CPA) is a neurotoxin that acts through inhibition of the sarco(endo)plasmic retic...
Enzymes play critical roles in the regulation of cellular function and are implicated in numerous di...
Enzymes play critical roles in the regulation of cellular function and are implicated in numerous di...
Enzymes play critical roles in the regulation of cellular function and are implicated in numerous di...
Enzymes play critical roles in the regulation of cellular function and are implicated in numerous di...
Type II restriction enzymes are homodimeric systems that bind four to eight base pair palindromic re...
AbstractThe synthetic substrate anthraniloyl-β-glycerol-P binds to myoinositol monophosphatase with ...