Four size exclusion chromatography (SEC) calibration techniques were tested for use in the molecular weight characterisation of Streptococcal Hyaluronic Acid (HA). An exponential equation, evaluated using the Hamielec method, was superior to the customary peak position method. It provided the most accurate estimates of the weight average molecular weight, Mw. The calibration was valid for HA in the range 800 - 2500 kDa, and permitted the calculation of both polydispersity and molecular weight distributions for HA from Streptococcal fermentations. This exponential calibration approach should have application in the characterisation of other large biopolymers, particularly where pore size of available SEC media is limiting
Size-exclusion chromatography (SEC) separates polymers by hydrodynamic volume (the universal calibra...
Size-exclusion chromatography (SEC) separates polymers by hydrodynamic volume (the universal calibra...
Objectives Because of the less studies in this field in Iraq, this study aimed to use local Streptoc...
The hyaluronic acid (HA) global market growth can be attributed to its use in medical, cosmetic, and...
Hyaluronic acid, HA, is a naturally occurring linear polysaccharide comprised of N-acetyl glucosamin...
The influence of three exptl. parameters, temp., pH and ionic strength of the eluent, on the retenti...
GAGs are produced by extraction and purification from different animal tissues. These preparations a...
A size exclusion chromatography (SEC) method has been established for the study of hyaluronic acid d...
The biological function of hyaluronic acid (HA) – the polysaccharide, which consists of repeating un...
Hyaluronic acid (HA) is polysaccharide widely used in the medicine. In the usage of HA the size of ...
The molecular weight (MW) of heparin can be accurately determined by size exclusion chromatography u...
We review here the advances that have been made in methodology using the analytical ultracentrifuge ...
The application possibilities of preparative size exclusion chromatography for the detailed analysis...
Size exclusion chromatography (SEC) is one of the most used analytical methods in chemistry to deter...
Hyaluronic acid (HA) is a linear, unbranched polymer, composed by simple disaccharide units, whose m...
Size-exclusion chromatography (SEC) separates polymers by hydrodynamic volume (the universal calibra...
Size-exclusion chromatography (SEC) separates polymers by hydrodynamic volume (the universal calibra...
Objectives Because of the less studies in this field in Iraq, this study aimed to use local Streptoc...
The hyaluronic acid (HA) global market growth can be attributed to its use in medical, cosmetic, and...
Hyaluronic acid, HA, is a naturally occurring linear polysaccharide comprised of N-acetyl glucosamin...
The influence of three exptl. parameters, temp., pH and ionic strength of the eluent, on the retenti...
GAGs are produced by extraction and purification from different animal tissues. These preparations a...
A size exclusion chromatography (SEC) method has been established for the study of hyaluronic acid d...
The biological function of hyaluronic acid (HA) – the polysaccharide, which consists of repeating un...
Hyaluronic acid (HA) is polysaccharide widely used in the medicine. In the usage of HA the size of ...
The molecular weight (MW) of heparin can be accurately determined by size exclusion chromatography u...
We review here the advances that have been made in methodology using the analytical ultracentrifuge ...
The application possibilities of preparative size exclusion chromatography for the detailed analysis...
Size exclusion chromatography (SEC) is one of the most used analytical methods in chemistry to deter...
Hyaluronic acid (HA) is a linear, unbranched polymer, composed by simple disaccharide units, whose m...
Size-exclusion chromatography (SEC) separates polymers by hydrodynamic volume (the universal calibra...
Size-exclusion chromatography (SEC) separates polymers by hydrodynamic volume (the universal calibra...
Objectives Because of the less studies in this field in Iraq, this study aimed to use local Streptoc...