Seminal plasma proteins and their relationship with percentage of morphologically normal sperm in 2-year-old Brahman (Bos indicus) bulls
- Boe-Hansen G.B.
- Rego J.P.A.
- Crisp, J.M.
- Moura, A.A.
- Nouwens, A.S.
- Li, Y.
- Venus, B.
- Burns, B.M.
- McGowan, M.R.
Publication date
November 2015
DOI Publisher
Elsevier BV ISSN
0378-4320 Citation count (estimate)
7Abstract
The objective was to determine the relationship between seminal plasma proteins and sperm morphology in Bos indicus bulls of the Brahman breed. Fifty-six 24-month-old Australian Brahman bulls were electroejaculated and samples were examined to determine the percentage of morphologically normal sperm (PNS24) and the seminal plasma protein composition was identified and quantified by 2-D gel electrophoresis. The total integrated optical density of 152 seminal plasma protein spots (SPPs) across all gels was determined using the PDQuest software version 8.0 (Bio Rad, USA). Using a single regression mixed model with the density of individual spots as a covariate for PNS24, 17 SPPs were significantly associated with PNS24 (
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