Aims/hypothesis: We used the db/db mouse to determine the nature of the secretory defect in intact islets. Methods: Glucose tolerance was compared in db/db and wild-type (WT) mice. Isolated islets were used: to measure insulin secretion and calcium in a two-photon assay of single-insulin-granule fusion; and for immunofluorescence of soluble N-ethylmaleimide-sensitive factor attachment proteins (SNAREs). Results: The 13-18-week-old db/db mice showed a diabetic phenotype. Isolated db/db islets showed a 77% reduction in insulin secretion induced by 15 mmol/l glucose and reductions in the amplitude and rise-time of the calcium response to glucose. Ionomycin-induced insulin secretion in WT but not db/db islets. Immunofluorescence showed an incre...
<div><p>Studying the response of islet cells to glucose stimulation is important for understanding c...
OBJECTIVE—The aim of the study was to elucidate the cellular mechanism underlying the suppression of...
The mechanism by which protein malnutrition impairs glucose-stimulated insulin secretion in the panc...
Aims/hypothesis In dispersed single beta cells the response of each cell to glucose is heterogeneous...
Aims/hypothesis We determined whether high-glucose-induced beta cell dysfunction is associated with ...
<p>(<b>A</b>) Insulin secretion from 4 sets of 5 islets each (each set from a different mouse, n = 2...
OBJECTIVE: The aim of the study was to elucidate the cellular mechanism underlying the suppression o...
Type 2 diabetes is characterized by islet dysfunction resulting in hyperglycemia, which can then lea...
OBJECTIVE The aim of the study was to elucidate the cellular mechanism underlying the suppression of...
<p>(<b>A</b>) Representative [Ca<sup>2+</sup>]<sub>cyt</sub> tracings in islets isolated from WT and...
Insulin-dependent diabetes mellitus (IDDM) is characterized by a progressive autoimmune destruction ...
Background/Aims: Rodent islets are often used for basic science research but they do not always reca...
Studying the response of islet cells to glucose stimulation is important for understanding cell func...
Type 2 diabetes is characterized by islet dysfunction resulting in hyperglycemia, which can then lea...
Studying the response of islet cells to glucose stimulation is important for understanding cell func...
<div><p>Studying the response of islet cells to glucose stimulation is important for understanding c...
OBJECTIVE—The aim of the study was to elucidate the cellular mechanism underlying the suppression of...
The mechanism by which protein malnutrition impairs glucose-stimulated insulin secretion in the panc...
Aims/hypothesis In dispersed single beta cells the response of each cell to glucose is heterogeneous...
Aims/hypothesis We determined whether high-glucose-induced beta cell dysfunction is associated with ...
<p>(<b>A</b>) Insulin secretion from 4 sets of 5 islets each (each set from a different mouse, n = 2...
OBJECTIVE: The aim of the study was to elucidate the cellular mechanism underlying the suppression o...
Type 2 diabetes is characterized by islet dysfunction resulting in hyperglycemia, which can then lea...
OBJECTIVE The aim of the study was to elucidate the cellular mechanism underlying the suppression of...
<p>(<b>A</b>) Representative [Ca<sup>2+</sup>]<sub>cyt</sub> tracings in islets isolated from WT and...
Insulin-dependent diabetes mellitus (IDDM) is characterized by a progressive autoimmune destruction ...
Background/Aims: Rodent islets are often used for basic science research but they do not always reca...
Studying the response of islet cells to glucose stimulation is important for understanding cell func...
Type 2 diabetes is characterized by islet dysfunction resulting in hyperglycemia, which can then lea...
Studying the response of islet cells to glucose stimulation is important for understanding cell func...
<div><p>Studying the response of islet cells to glucose stimulation is important for understanding c...
OBJECTIVE—The aim of the study was to elucidate the cellular mechanism underlying the suppression of...
The mechanism by which protein malnutrition impairs glucose-stimulated insulin secretion in the panc...