Detection of a novel human coronavirus by real-time reverse-transcription polymerase chain reaction
- Corman, V.M. (Victor)
- Eckerle, I.
- Bleicker, T.
- Zaki, A.
- Landt, O.
- Eschbach-Bludau, M. (Monika)
- Boheemen, S. (Sander) van
- Gopal, R. (Robin)
- Ballhause, M.
- Bestebroer, T.M. (Theo)
- Muth, D.
- Müller, M.A. (Marcel)
- Drexler, J.-F. (Jan-Felix)
- Zambon, M.C. (Maria)
- Osterhaus, A.D.M.E. (Albert)
- Fouchier, R.A.M. (Ron)
- Drosten, C. (Christian)
Publication date
September 2012
DOI ISSN
1560-7917 Citation count (estimate)
252Abstract
We present two real-time reverse-transcription polymerase chain reaction assays for a novel human coronavirus (CoV), targeting regions upstream of the E gene (upE) or within open reading frame (ORF)1b, respectively. Sensitivity for upE is 3.4 copies per reaction (95% confidence interval (CI): 2.5-6.9 copies) or 291 copies/mL of sample. No cross-reactivity was observed with coronaviruses OC43, NL63, 229E, SARS-CoV, nor with 92 clinical specimens containing common human respiratory viruses. We recommend using upE for screening and ORF1b for confirmation
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