Soluble, membrane and wall peroxidases, phenylalanine ammonia-lyase, and lignin changes in relation to vitrification of carnation tissues cultured in vitro

Vitrification of carnation tissues by passage from solid to liquid medium was accompanied by decreased lignin content. The higher lignin level of normal tissues corresponded to a higher phenylalanine ammonia-lyase activity. It also paralleled a much higher activity of soluble and wall peroxidases towards syringaldazine. Kinetical analysis of soluble isoperoxidases indicated a two-step control of basic and acidic enzymes with a diminished activity of the latter in glassy plants. Compartment analysis of guaiacol peroxidases showed a different distribution in malformed tissues