Transcriptome Profiling Of Feeding-To-Fasting Transition In Chicken Liver Using A Chicken 20K Oligo Microarray

International audienceStarvation triggers a complex array of adaptative metabolic responses including energy-metabolic responses, a process which must imply tissue specific alterations in gene expression profiles. In the chicken, liver is a major organ controlling energy metabolism. The present study aimed to describe the evolution of global gene expression profiles in chicken liver during a 48h fasting period. Liver RNA samples were collected from 4 weeks old broilers, fed ad libitum or fasted for 16h or 48h. Following reverse-transcription and Cy dye labelling, the samples were hybridized on chicken 20K oligochips (ARK-genomics) against a reference sample. The data were then normalized by “Lowess-fitness” and analyzed by analysis of variance using LIMMA package. The number of genes altered by fasting increased from 190 at 16h to 611 at 48h (p