Removal of empty capsids from adeno-associated virus (AAV) manufacturing lots remains a critical step in the downstream processing of AAV clinical-grade batches. Because of similar physico-chemical characteristics, the AAV capsid populations totally lacking or containing partial viral DNA are difficult to separate from the desired vector capsid populations. Based on minute differences in density, ultracentrifugation remains the most effective separation method and has been extensively used at small scale but has limitations associated with availabilities and operational complexities in large-scale processing. In this paper, we report a scalable, robust, and versatile anion-exchange chromatography (AEX) method for removing empty capsids and ...
Teschner J. Improvement of rAAV production by analysis of capsid modification, producer cell line ge...
Adeno-associated viral (AAV) vectors have emerged as one of the most popular gene transfer systems i...
Ion-exchange chromatography coupled to light scattering detectors represents a fast and simple analy...
Gene therapy is opening unprecedented opportunities for novel therapeutic approaches. Based on the c...
Recombinant adeno-associated virus serotype 9 (rAAV9) can specifically transduce muscle and neuronal...
The generation of clinical good manufacturing practices (GMP)-grade adeno-associated virus (AAV) vec...
Adeno-associated virus vector manufacturing at scale continues to hinder the application of AAV tech...
We describe a new rapid, low cost, and scalable method for purification of various recombinant adeno...
Recombinant adeno-associated virus (rAAV) is a mammalian virus that has been altered to be used as a...
Recombinant adeno-associated virus (AAV) is a valuable and often used gene therapy vector. With incr...
Adeno-associated virus (AAV) is a strong candidate for single-gene mutation gene therapy. AAV comes ...
Adeno-associated virus (AAV) is one of the most promising gene therapy vectors and is widely used as...
Manufacturing practices for recombinant adeno-associated viruses (AAV) have improved in the last dec...
Adeno-associated virus (AAV) is an increasingly popular tool in the research laboratory, and use of ...
Adeno-associated virus is being developed as a human gene therapy vector. Recombinant AAV vectors ar...
Teschner J. Improvement of rAAV production by analysis of capsid modification, producer cell line ge...
Adeno-associated viral (AAV) vectors have emerged as one of the most popular gene transfer systems i...
Ion-exchange chromatography coupled to light scattering detectors represents a fast and simple analy...
Gene therapy is opening unprecedented opportunities for novel therapeutic approaches. Based on the c...
Recombinant adeno-associated virus serotype 9 (rAAV9) can specifically transduce muscle and neuronal...
The generation of clinical good manufacturing practices (GMP)-grade adeno-associated virus (AAV) vec...
Adeno-associated virus vector manufacturing at scale continues to hinder the application of AAV tech...
We describe a new rapid, low cost, and scalable method for purification of various recombinant adeno...
Recombinant adeno-associated virus (rAAV) is a mammalian virus that has been altered to be used as a...
Recombinant adeno-associated virus (AAV) is a valuable and often used gene therapy vector. With incr...
Adeno-associated virus (AAV) is a strong candidate for single-gene mutation gene therapy. AAV comes ...
Adeno-associated virus (AAV) is one of the most promising gene therapy vectors and is widely used as...
Manufacturing practices for recombinant adeno-associated viruses (AAV) have improved in the last dec...
Adeno-associated virus (AAV) is an increasingly popular tool in the research laboratory, and use of ...
Adeno-associated virus is being developed as a human gene therapy vector. Recombinant AAV vectors ar...
Teschner J. Improvement of rAAV production by analysis of capsid modification, producer cell line ge...
Adeno-associated viral (AAV) vectors have emerged as one of the most popular gene transfer systems i...
Ion-exchange chromatography coupled to light scattering detectors represents a fast and simple analy...