OPTIMASI VOLUME PELARUT DAN WAKTU MASERASI PENGAMBILAN FLAVONOID DAUN BELIMBING WULUH

Bilimbi is one of the plants that contains flavonoids and it can be found in Indonesia, however its utilization is not optimal yet. Flavanoids are known as one of the largest natural phenols which has functions not only as an antioxidant but also can protect the cell structure, improve the effectiveness of vitamin C, antiinflammatory, preventing bone loss, antidiarrheal, antidiabetic and antibiotics. Some previous research is focused on testing the effectiveness of flavonoids as traditional medicine, but there is no research evaluating the optimal conditions of flavonoids extraction from bilimbi’s leaves. This research aims to determine the optimal conditions which include the volume of solvent and maceration time, in order to obtain the most optimal of flavonoids extracted and are expected can improve its effectiveness as materials for traditional medicine in the future. The research begin with the preparation of materials. Then, 10 grams of bilimbi’s leaves were macerated using ethanol 96% by volume solvent and maceration time varied. Before the maceration time is calculated, stirring with a mercury stirrer speed of 200 rpm for 1 hour. Liquid extract of maceration is filtered by using a buchner funnel, then concentrated by using rotary evaporator. Condensed extract was analyzed quantitatively by using UV-Vis spectrophotometer. Optimal conditions of this research was obtained when using volume of solvent 250 mL and macerated for 48 hours which 72.31 mg of flavonoids extracted as its result