Influence of the coordinative copper compounds on the activity of liver lisosomal proteases in propylene glycol poisoning

Laborator biochimie USMF “N.Testemiţanu”We studied the influence of copper coordinative compounds CMT-28 and CMT-67 and their combination with cyanobacterial remedy BioR on hepatic lysosomal proteases activity in intoxication with propylene glycol (PG). It was established that PG enhance statistically conclusive the activity of cathepsines D, G, L, H, B and leucinaminopeptidase in rat liver. All studied remedies in low doses (1 g/kg) reduced statistically reliable leucineaminopeptidase activity, CMT-28 and CMT-67+BioR influenced cathepsin H activity and CMT-67+BioR - cathepsin L activity. In high doses (10 mg/kg) statistically conclusive was reduced only the activity of cathepsin G by CMT-67, CMT-67+BioR, CMT-28+BioR and cathepsin H activity by CMT-28 + BioR. S-a studiat influenţa compuşilor coordinative ai cuprului CMT-28 şi CMT-67, precum şi a combinaţiilor lor cu remediul cianobacterian BioR asupra activităţii proteazelor lizozomale hepatice în intoxicaţia cu propilenglicol (PG). S-a stabilit că PG amplifică statistic concludent activitatea catepsinelor D, G, L, H, B şi a leucinaminopeptidazei în ficatul şobolanilor. În doze mici (1 mg/kg) toate preparatele studiate diminuează statistic veridic activitatea leucinamino-peptidazei, CMT-28 şi CMT-67 + BioR influenţează activitatea catepsinei H, iar CMT-67 + BioR – activitatea catepsinei L. În doze mari (10 mg/kg) este micşorată statistic veridic doar activitatea catepsinei G de către CMT-67, CMT-67 + BioR şi CMT-28 + BioR) şi a catepsinei H de către CMT-28 + BioR