The activity of renal lysosomal hydrolases in ethylene glycol intoxication and the influence of some new local biologically active compounds

Laboratorul ştiinţific Biochimie, USMF „Nicolae Testemiţanu”The influence of some new local biologically active compounds (BAC) on renal lysosomal hydrolases (RLH) in ethylene glycol (EG) poisoning was investigated. The tested BAC demonstrated a pronounced tropic effect on lysosomes, influencing in different ways the activity of RLH and modifying the functional status of lysosomal apparatus. The complex compounds − CMD-4, CMD-8 and CMJ-23, remedies BioR and BioR-Ge significantly reduced the functionality of cathepsins B, H and of elastase. CMJ-23 decreased the activity of cathepsin G, b-glucosidase, arylsulfatases A, B and C, which showed the high responsiveness of these hydrolases to the action of BAC. CMD-8 increased the activity of cathepsin D, b-glucosidase and of N-acetyl-β-D-glucosaminidase. The remedy BioR normalized the activity of elastase, while BioR-Ge increased the acid phosphatase. The increased activity of RLH in the administration of the tested BAC, can be considered as a reaction of compensation-adaptation of the body, aimed to enhance the biodegradation of damaged molecules resulted from the toxic action of EG and of its metabolic products.S-a cercetat influenţa unor compuşi biologici activi (CBA) autohtoni noi asupra activităţii hidrolazelor lizozomale renale (HLR) în intoxicaţia cu etilenglicol (EG). CBA testaţi au demonstrat un lizozomotropism pronunţat, fiind capabili să influenţeze în mod diferit activitatea HLR şi să modifice starea funcţională a aparatului lizozomal. Compuşii complecşi – CMD-4, CMD-8 şi CMJ-23, remediile BioR, BioR-Ge au redus pregnant nivelul funcţional al catepsinelor B, H şi elastazei. CMJ-23 a diminuat activitatea catepsinei G, b-glucozidazei, arilsulftazelor A, B şi C., fapt ce a demonstrat sensibilitatea înaltă a acestor hidrolaze la acţiunea CBA. CMD-8 a majorat activitatea catepsinei D, b-glucozidazei şi N-acetil-β-D-glucozaminidazei. Remediul BioR a normalizat enzimoactivitatea elastazei, iar BioR-Ge a crescut fosfataza acidă. Intensificarea activităţii HLR la administrarea CBA cercetate, poate fi apreciată ca o reacţie compensatoare de adaptare a organismului, ce tinde să intensifice biodegradarea moleculelor defectuoase ce se formează în rezultatul acţiunii toxice a EG şi a produşilor lui de metabolizare