Measurement of luminescence intensity on textiles using Luminous bacterial biocatalytic system

Nature is the most exquisite thing around us with the existence of living organisms exhibiting different phenomena such as water repel/ency, touch sensitive plant and chameleon skin. Some of these phenomena inspired scientists to explore and design smart fabrics biomimicking the behaviour or pattern in living organisms. Bioluminescence is one such phenomenon where-in different living organisms such as firefly, jelly fish and crustaceans have the ability to impart visible light of specific wavelength, by enzyme catalysed reactions. Existence and study of such light emitting living organisms have been carried out, and harnessing these reactions has already transformed significant areas of medical field and clinical diagnosis, but research work on transforming this into living light is limited. In the present study, luminous bacterial system was investigated to assess and detect the bioluminescence behaviour onto the textile material. In the Luminous bacterial system, in vivo biochemical mecha­nism involves two different enzymes as well as different substrate components. Emission of light due to in vivo luminous bacterial reaction mechanism is seen in visible region. For in vitro reaction mechanism study, physical adsorption technique was used to graft both enzymes on plasma activated PET nonwoven textile and when substrates were introduced manually during the analysis, the biochemical reaction leading to light production occured. A Luminometer equipment was used to determine the light intensity in terms of Relative light units (RLU). The measurement results were obtained for nonwoven plasma treated PET with enzyme and substrate addition at different concentration and RLU value was obtained. The analysis data revealed that light intensity in RLU could be recorded by introducing both the enzymes and substrates on textile material, however intensive research is required in order to observe emitted light through the naked eye. The research study will help to attai