Antidotal effects of thiol containing compounds with a special study of a method for the detection of BAL

(1) A new method for estimation of BAL in - vitro and ir vivo is described depending on the enzyme disinhibition method.(2) The method is sensitive and delicate and can detect BAL up to a fraction of μg. depending on the urease employed.(3) The method is not specific far dithiols as monothiols thio-glycollic acid and cysteine hydro-chloride also give positive test.(4) The 'method is equally applicable for estimation of thiols in biological fluids, e.g., in blood and urine.(5) Evidence has been obtained that normal plasma contains -SH groups which interfere with the poisoning activity of mercury.(6) The fate of various di- and monothiols in blood has been studied after intramuscular administration of various thiols in rabbits.(7) ) Further evidence in support of.detoxifying capacity of liver for BAL has been put forward by comparing the blood concentration of BAL (20 mg.kg.) in normal and in liver damaged rabbits.(B) Excretion of BAL in urine after intramuscular injection of 50 mg.BAL/kg. has been studied, and the possibility of tubular excretion of BAL in experimental animals has teen put forward.(9) The excretion of BAL in renal damaged rabbits could not be studied by this method as uranylion employed for renal damage inactivates BAL