Immunoliposomes in haematology

The aim of this -thesis was to use antibody-directed liposomes to enhance the targeting of 1. fluorescent compounds for the detection of cell surface antigens and 2. cytotoxic compounds for delivery to specific cell populations. This involved the selection and production of a suitable species of 1ipoaome-protein conjugate from among those described in the literature. The choice was a small unilamellar liposome conjugated either to antibody or protein A via a covalent linkage mediated by the heterobifunctional reagent SPDP.Anti-immunoglobulin antibody was a more universal ligand than either specific antibody or protein A. The conjugate was made fluorescent by the encapsulation of carboxyfluorescein