Determination of pharmaceuticaly important substances using High Performance Liquid Chromatography with Mass Spectrometry detection

High performance liquid chromatography (HPLC) in combination with Mass Spectrometry (MS) is frequently used for the determination of drugs in human plasma and it belongs to group of methods that enable new and modern modifications. Highly sensitive methods that do not require time consuming sample pre-treatment are desired for pharmacokinetic (PK) studies. Double MS (MS/MS) detection provides drugs identification and high sensitivity for quantitative determination. It effectively eliminates interferences from endogenous impurities and guarantees method selectivity at the same time. PK studies determine pharmacokinetic characteristics of particular drug that are then used to assess the bioequivalence of determined drug versus other chosen drug of the same nature. The PK parameters are calculated from the plasma concentrations of the drug determined by a validated method. The aim of my thesis was to show the use of HPLC-MS/MS methods in pharmacokinetic studies. All developed methods were successfully employed in the pharmacokinetic studies to assess the pharmacokinetic parameters. A validated, highly sensitive, and selective HPLC method with MS-MS detection has been developed for quantitative determination of azithromycin (AZI) in human Na2EDTA plasma. Roxithromycin (ROX) was used as internal..