Evaluation of properties of new HILIC stationary phases for pteridins using UHPLC-FD method

Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of analytical chemistry Candidate: Iva Kaufmannová Supervisor: PharmDr. Lucie Nováková, Ph.D. Title of Doctoral Thesis: Evaluation of properties of new HILIC stationary phases for pteridins using UHPLC-FD method The subject of this thesis was to evaluate the characteristics of three new types of HILIC stationary phases for the identification and quantification of biologically active substances biopterin, neopterin, dihydrobiopterin and dihydroneopterin by UHPLC with fluorescence detection. Neopterin is used as a marker of immune system activation and inflammatory diseases. Its early detection in urine or plasma may indicate a pathological immune activity. Elevated concentration of neopterin is described in viral or bacterial diseases, in autoimmune diseases in HIV infection or in malignant tumors. 7,8 - dihydroneopterin is able to prevent proteins and lipoproteins from oxidative damage. Hydrophilic Interaction Liquid Chromatography (HILIC) is a chromatographic method that can be used to improve retention of very polar compounds. It uses a polar stationary phase and mobile phase containing a certain amount of water and polar solvents. UHPLC is the most advanced separation technology to develop a pressure of about 100 MPa...