Biosynthesis of sterols, which are considered essential components of virtually all eukaryotic membranes, requires molecular oxygen. Anaerobic growth of the yeast Saccharomyces cerevisiae therefore strictly depends on sterol supplementation of synthetic growth media. Neocallimastigomycota are a group of strictly anaerobic fungi which, instead of containing sterols, contain the pentacyclic triterpenoid "sterol surrogate" tetrahymanol, which is formed by cyclization of squalene. Here, we demonstrate that expression of the squalene-tetrahymanol cyclase gene TtTHC1 from the ciliate Tetrahymena thermophila enables synthesis of tetrahymanol by S. cerevisiae. Moreover, expression of TtTHC1 enabled exponential growth of anaerobic S. cerevisiae cult...
The yeast Saccharomyces cerevisiae possesses the endogenous precursor for diterpene production but d...
AbstractFatty acyl esters of the yeast specific sterol, ergosterol, are exclusively stored in lipid ...
Abstract Steroidal compounds are of great interest in the pharmaceutical field, with steroidal drugs...
Biosynthesis of sterols, which are considered essential components of virtually all eukaryotic membr...
Biosynthesis of sterols, which are key constituents of canonical eukaryotic membranes, requires mole...
Abstract The molecular mechanisms that govern intracellular transport of sterols in eukaryotic cells...
Current large-scale, anaerobic industrial processes for ethanol production from renewable carbohydra...
Sterols are important molecules in regulating membrane fluidity and permeability in eukaryotic cells...
It could be established that the total sterol content is an individual and reproducible characterist...
The sterol biosynthetic pathway is essential for growth of all eukaryotic cells and the main target ...
Yeast is the most primitive organism synthesizing substantial amounts of sterols. Because of this eu...
Saccharomyces cerevisiae is one of the few yeast species that can grow equally well without molecula...
The molecular mechanisms that govern intracellular transport of sterols in eukaryotic cells are only...
Sterols are essential components of eukaryotic membranes and contribute to important physiological c...
AbstractCertain exogenously-supplied sterols, like ergost-8-enol, are efficiently converted into erg...
The yeast Saccharomyces cerevisiae possesses the endogenous precursor for diterpene production but d...
AbstractFatty acyl esters of the yeast specific sterol, ergosterol, are exclusively stored in lipid ...
Abstract Steroidal compounds are of great interest in the pharmaceutical field, with steroidal drugs...
Biosynthesis of sterols, which are considered essential components of virtually all eukaryotic membr...
Biosynthesis of sterols, which are key constituents of canonical eukaryotic membranes, requires mole...
Abstract The molecular mechanisms that govern intracellular transport of sterols in eukaryotic cells...
Current large-scale, anaerobic industrial processes for ethanol production from renewable carbohydra...
Sterols are important molecules in regulating membrane fluidity and permeability in eukaryotic cells...
It could be established that the total sterol content is an individual and reproducible characterist...
The sterol biosynthetic pathway is essential for growth of all eukaryotic cells and the main target ...
Yeast is the most primitive organism synthesizing substantial amounts of sterols. Because of this eu...
Saccharomyces cerevisiae is one of the few yeast species that can grow equally well without molecula...
The molecular mechanisms that govern intracellular transport of sterols in eukaryotic cells are only...
Sterols are essential components of eukaryotic membranes and contribute to important physiological c...
AbstractCertain exogenously-supplied sterols, like ergost-8-enol, are efficiently converted into erg...
The yeast Saccharomyces cerevisiae possesses the endogenous precursor for diterpene production but d...
AbstractFatty acyl esters of the yeast specific sterol, ergosterol, are exclusively stored in lipid ...
Abstract Steroidal compounds are of great interest in the pharmaceutical field, with steroidal drugs...