VARIABILITY OF MITOCHONDRIAL CYTOCHROME OXYDASE SUBUNIT I GENE SEQUENCE IN SPECIES OF THE GENERA AEDES AND OCHLEROTATUS (DIPTERA: CULICIDAE)

N. V. Khrabrova
Yu. V. Andreeva
O. V. Vaulin
S. S. Alekseeva
A. K. Sibataev

Publication date

December 2014

Publisher

Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders

Journal

Vavilov Journal of Genetics and Breeding

Abstract

Sequence variation in the 538-bp fragment from the 5′ end of the COI region was analyzed to test its usefulness in the identification of 15 mosquito species of the genera Aedes and Ochlerotatus (Diptera: Culicidae) from Tomsk and Kemerovo regions and 14 species from GenBank. The divergences between congeneric species averaged 7,8 % (from 0,7 to 13,1 %), whereas those for conspecific individuals averaged 0,57 % (0 to 1,6 %). The sequences for four species pairs (3,7 %) showed < 2 % divergence. In most cases, individuals of a single species grouped closely together, but deeper divergences were detected in two species (Och. euedes and Ae. vexans)