Effect of Plant Growth Regulators on in vitro Direct Plant Organogenesis of White Kelampayan (Neolamarckia cadamba)

White Kelampayan, its scientific name is Neolamarckia cadamba belongs to the family of Rubiaceae. The fast growing characteristic of this tree species was found to have potential to be planted commercially because of it widely usage in wood industry and reforestation but it is difficult and time consuming to propagate using conventional method. However, an alternative approach had been evaluated to replace the problem caused by difficulties in traditional vegetative propagation to plant regenerating for in vitro studies. In this study, an efficient system was developed for direct plant organogenesis from in vitro derived leaf explants of N. cadamba, without the formation of callus phase. The in vitro procedure involved three steps that included induction of shoot initials from leaf tissues, regeneration and elongation of shoots from the shoot initials. The medium used for induction of shoot was achieved on full strength Murashige and Skoog (MS) medium supplemented with combination of cytokinin and auxin in different concentration. Through this experiment, the highest percentage of shoots induction (25%) recorded was using MS medium with 3.0 mg/L BAP and 1.5 mg/L NAA, while the lowest percentage of shoots· induction (4.2 %) was recorded on the medium supplemented with 1.0 mg/L BAP and 1.5 mg/L NAA. The in vitro direct plant regenerated shoots showed the same morphological characteristics as mother plants