Jacob's Well is a karst spring that discharges from the Trinity aquifer in central Texas. Ongoing development and increased groundwater pumping in the area around the spring are thought to be impacting the volume of discharge at the spring--however, the source of spring discharge is poorly understood. The goals of this study are to characterize the chemistry of springflow and nearby surface waters and groundwaters and to identify the source or sources of water that contribute flow to the spring under low flow conditions. The spring is located in the Middle Trinity aquifer within the Lower Cretaceous Trinity Group in central Texas. The Trinity Group is composed of alternating terrestrial and marine deposits, with most groundwater flow occur...