α-Amylase from Bacillus paralicheniformis (BliAmy), belonging to GH13_5 subfamily of glycoside hydrolases, was proven to be a highly efficient raw starch digesting enzyme. The ability of some α-amylases to hydrolyze raw starch is related to the existence of surface binding sites (SBSs) for polysaccharides that can be distant from the active site. Crystallographic studies performed on BliAmy in the apo form and of enzyme bound with different oligosaccharides and oligosaccharide precursors revealed binding of these ligands to one SBS with two amino acids F257 and Y358 mainly involved in complex formation. The role of this SBS in starch binding and degradation was probed by designing enzyme variants mutated in this region (F257A and Y358A). Ki...
Starch is a major storage product of many economically important crops such as wheat, rice, maize, t...
AbstractCertain starch hydrolases possess secondary carbohydrate binding sites outside of the active...
α-Amylases from Bacillus sp. strains digesting raw starch were observed to adsorb to raw starch and ...
α-Amylase from Bacillus paralicheniformis (BliAmy), belonging to GH13_5 subfamily of glycoside hydro...
Some polysaccharide processing enzymes possess secondary carbohydrate binding sites situated on the ...
Germinating barley seeds contain multiple forms of α-amylase, which are subject to both differential...
Starch provides the major source of caloric intake in many diets. Cleavage of starch into malto-olig...
α-Amylase is one of the important enzymes in the starch-processing industry. However, starch process...
α-Amylases (EC 3.2.1.1) hydrolyze internal α-1,4-glucosidic linkages of starch and related glucans. ...
α-Amylases occur widely in plants, animals, and microorganisms. They often act in synergy with other...
AbstractA novel starch-binding domain (SBD) that represents a new carbohydrate-binding module family...
Abstract Background β-amylase (EC 3.2.1.2) is an exo-enzyme that shows high specificity for cleaving...
Amylases catalyze the hydrolysis of starch material and play central roles in carbohydrate metabolis...
AbstractBackground: Carbohydrate-binding domains are usually small and physically separate from the ...
Starch is the main energy store of major agricultural crops such as corn, potato, rice and wheat. Va...
Starch is a major storage product of many economically important crops such as wheat, rice, maize, t...
AbstractCertain starch hydrolases possess secondary carbohydrate binding sites outside of the active...
α-Amylases from Bacillus sp. strains digesting raw starch were observed to adsorb to raw starch and ...
α-Amylase from Bacillus paralicheniformis (BliAmy), belonging to GH13_5 subfamily of glycoside hydro...
Some polysaccharide processing enzymes possess secondary carbohydrate binding sites situated on the ...
Germinating barley seeds contain multiple forms of α-amylase, which are subject to both differential...
Starch provides the major source of caloric intake in many diets. Cleavage of starch into malto-olig...
α-Amylase is one of the important enzymes in the starch-processing industry. However, starch process...
α-Amylases (EC 3.2.1.1) hydrolyze internal α-1,4-glucosidic linkages of starch and related glucans. ...
α-Amylases occur widely in plants, animals, and microorganisms. They often act in synergy with other...
AbstractA novel starch-binding domain (SBD) that represents a new carbohydrate-binding module family...
Abstract Background β-amylase (EC 3.2.1.2) is an exo-enzyme that shows high specificity for cleaving...
Amylases catalyze the hydrolysis of starch material and play central roles in carbohydrate metabolis...
AbstractBackground: Carbohydrate-binding domains are usually small and physically separate from the ...
Starch is the main energy store of major agricultural crops such as corn, potato, rice and wheat. Va...
Starch is a major storage product of many economically important crops such as wheat, rice, maize, t...
AbstractCertain starch hydrolases possess secondary carbohydrate binding sites outside of the active...
α-Amylases from Bacillus sp. strains digesting raw starch were observed to adsorb to raw starch and ...