Evaluation of six methylation markers derived from genome-wide screens for detection of cervical precancer and cancer

Aim:To evaluate the triage performance of six host-cell DNA methylation markers derived from two genome-wide discovery screens for detection of cervical precancer (cervical intraepithelial neoplasia 3 [CIN]) and cancer.Materials & methods:Human papillomavirus-positive cervical scrapes of controls (0.800 after leave-one-out cross-validation. Bi-marker panelASCL1/LHX8had highest area under the curve (0.882), and detected 83.4% of CIN3 and all cervical cancers at specificity of 82.4%.Conclusion:All six methylation markers showed an equivalent, high performance for the triage of human papillomavirus-positive women using cervical scrapes with complementarity between markers