Identifikasi Senyawa isolasi Akar Pasak Bumi(Eurycoma longifolia Jack) Secara Spektrofotometri Dan Uji Sitotoksisitas Terhadap Terdapat Sel Hela

Eurycoma longifolia Jack is one of Indonesia native plants that have anti-cancer potential. Isolated coumpouns methanol extract of the roots of Pasak Bumi in a preliminary study proved to be active as anti-angiogenesis, so that further research needs to be done to prove the potential cytotoxicty the celllinien vitroin an attempt to find new drugs for both cancer chemo preventive or chemotherapy.Approach also needs to be done to the structur of the isolated compouds roots of pasak bumi (Eurycoma longifolia Jack)toensure that types of compounds as anti-cancer potential. The roots of pasak bumi powder macerated with methanol and then fractionated using Vacuum Liquid Chromatograpy (VLC). Fraction to 2 which fluoresces blue under UV254 nm is purificated using preparative TCL, the resulting isolates the test compound. In vitro cytotoxicty assay with Hela cells made to isolate the test compoundand using the positive control doxorubicin. Identification by UV and IR spectroscopy showed that the isolates isolated compounds have pasak bumi roots chomophore group and functional group C=O,-OH,-CH2,_CH3,C=N and is able to inhibit the growth of Hela cells but low potential. Keyword:the roots of pasak bumi, the cytotoxictest, Hela cell