Efficient single-step rapeseed oleosome extraction using twin-screw press

Oil in seeds is encapsulated in oleosomes, which are small lipid droplets surrounded by a phospholipid-protein monolayer. The currently proposed method to extract intact oleosomes includes mixing seeds with alkaline media in a ratio 1:7, batch blending and filtering. In this work, we propose the use of a twin-screw press to perform the oleosome extraction at pH 7. The results show that similarly to blender extraction, twin-screw press recovers ⁓60% of the oleosomes; however the twin-screw press is able to achieve this yield even when just pure water is used. While in the blender extraction, the yield depends on ionic strength and pH of the extraction media, when using twin-screw press, the oleosome extraction yield predominantly depends on the mechanical forces. These shear forces are able to break the cell walls and release the cellular material while maintaining the integrity of oleosomes. The oleosomes extracted with twin-screw press have similar characteristics than those obtained by the blending process. Overall, twin-screw press seems a promising alternative to scale-up the oleosome aqueous extraction, especially as neutral pH can be used and the water usage is significantly reduced. Additionally, preliminary results showed that the yield can increase up to 90 wt%