Cis Regulatory Elements (CREs) regulate the expression of the genes in their genomic neighborhoods and influence cellular processes such as cell-fate maintenance and differentiation. To date, there remain major gaps in the functional characterization of CREs and the identification of its target genes in the cellular native environment. In this study, we performed a Features Oriented CRISPR Utilized Systematic (FOCUS) screen of OCT4-bound CREs using CRISPR/Cas9 to identify functional enhancers important for pluripotency maintenance in mouse ES cells. From the initial 235 candidates tested, 16 CREs were identified to be essential stem cell enhancers. Using RNA-seq and genomic 4C-seq, we further uncovered a complex network of candidate CREs an...
The pluripotency of embryonic stem cells (ESCs) is maintained by a small group of master transcripti...
Poised enhancers marked by H3K27me3 in pluripotent stem cells have been implicated in the establishm...
With <2% of the human genome coding for proteins, a major challenge is to interpret the function ...
cis-regulatory elements (CREs) regulate the expression of genes in their genomic neighborhoods and i...
Regulatory elements (REs) consist of enhancers and promoters that occupy a significant portion of th...
Cis-regulatory elements (CREs) orchestrate the spatiotemporal control of gene expression. The regula...
Pluripotency maintenance and exit in embryonic stem cells is a focal topic in stem cell biology. How...
In vitro cell lines derived from the early embryo retain the identity and lineage restrictions of th...
. These authors contributed equally to this work. The pluripotency of embryonic stem cells (ESCs) is...
mbryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) hold great promise in regenera...
Poised enhancers marked by H3K27me3 in pluripotent stem cells have been implicated in the establishm...
The mechanisms underlying the gene regulation and enhancer-promoter rewiring which contribute to the...
<div><p>The pluripotency of embryonic stem cells (ESCs) is maintained by a small group of master tra...
2018-10-16Exploration of the genome and functional elements contained within the DNA sequence contin...
textabstractEnhancers are genetic elements that regulate spatiotemporal gene expression. Enhancer fu...
The pluripotency of embryonic stem cells (ESCs) is maintained by a small group of master transcripti...
Poised enhancers marked by H3K27me3 in pluripotent stem cells have been implicated in the establishm...
With <2% of the human genome coding for proteins, a major challenge is to interpret the function ...
cis-regulatory elements (CREs) regulate the expression of genes in their genomic neighborhoods and i...
Regulatory elements (REs) consist of enhancers and promoters that occupy a significant portion of th...
Cis-regulatory elements (CREs) orchestrate the spatiotemporal control of gene expression. The regula...
Pluripotency maintenance and exit in embryonic stem cells is a focal topic in stem cell biology. How...
In vitro cell lines derived from the early embryo retain the identity and lineage restrictions of th...
. These authors contributed equally to this work. The pluripotency of embryonic stem cells (ESCs) is...
mbryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) hold great promise in regenera...
Poised enhancers marked by H3K27me3 in pluripotent stem cells have been implicated in the establishm...
The mechanisms underlying the gene regulation and enhancer-promoter rewiring which contribute to the...
<div><p>The pluripotency of embryonic stem cells (ESCs) is maintained by a small group of master tra...
2018-10-16Exploration of the genome and functional elements contained within the DNA sequence contin...
textabstractEnhancers are genetic elements that regulate spatiotemporal gene expression. Enhancer fu...
The pluripotency of embryonic stem cells (ESCs) is maintained by a small group of master transcripti...
Poised enhancers marked by H3K27me3 in pluripotent stem cells have been implicated in the establishm...
With <2% of the human genome coding for proteins, a major challenge is to interpret the function ...