Prisustvo virusa žutice postrne repe na uljanoj repici (Brassica napus L.) u Srbiji

A total of 86 oilseed rape samples from six crops in different localities were collected during 2014 and analyzed for the presence of Turnip yellows virus (TuYV), Cauliflower mosaic virus (CaMV) and Turnip mosaic virus (TuMV) using commercial double-antibody sandwich (DAS)-ELISA kits. TuYV was serologically detected in 60 collected samples (69.77%), and none of the samples tested were positive for CaMV and TuMV. Six selected TuYV isolates were successfully transmitted by Myzus persicae to three test plants, confirming the infectious nature of the disease. In the selected ELISA-positive samples, the presence of TuYV was further confirmed by RT-PCR and sequencing. A comparison of the obtained sequence with those available in GenBank confirmed the presence of TuYV in oilseed rape samples. An analysis of P0 gene sequence data for a subset of these isolates showed they clustered with the known TuYV and were distinct from Beet western yellows virus (BWYV) isolates.Tokom 2014. godine, sa šest lokaliteta gajenja uljane repice, ukupno je sakupljeno 86 uzorka koji su serološki testirani na prisustvo virusa žutice postrne repe [Turnip yellow virus (TuYV)], virusa mozaika karfiola [Cauliflower mosaic virus (CaMV)] i virusa mozaika postrne repe [Turnip mosaic virus (TuMV)], korišćenjem komercijalno dostupnih kitova za DAS-ELISA test. Prisustvo TuYV dokazano je u 60 (69.77%) prikupljenih uzoraka, dok prisustvo CaMV i TuMV nije dokazano ni u jednom od testiranih uzoraka. Za dalja istraživanja odabrano je šest uzoraka prirodno zaraženih biljaka uljane repice poreklom iz različitih lokaliteta koji su uspešno preneti vašima na tri različite test biljke, čime je potvrđena infektivna priroda oboljenja. Prisustvo TuYV u ELISA pozitivním uzorcima je potvrđeno korišćenjem RT-PCR i sekvencioniranjem. Poređenjem dobijenih sekvenci sa sekvencama dostupnih u GenBank bazi podataka, potvrđena je autentičnost serološki detektovanih virusa. Na osnovu sekvenci P0 gena izolata TuYV, utvrđena je pripadnost ispitivanih izolata iz uljane repice grupi sa ostalim TuYV izolatima, koji su jasno razdvojeni od Beet western yellows virus (BWYV) izolata