Prisustvo zearalenona u najčešće uzgajanim sortama pšenice u Srbiji

A total of 45 samples of wheat from three different locations in Vojvodina were analyzed for the presence of zearalenone. Analytical methods based on clean-up by solid-phase extraction (SPE) columns and detection by liquid chromatography were used after validation. Limit of detection for ZEA in wheat was 18.6 μg/kg and the limit of quantification was 56.5 μg/kg. Recovery values ranged between 86% and 97%. The occurrence of ZEA in wheat was rather high with 53.3% of positive samples with the average value of 330 μg/kg. Incidences were found from 68 μg/kg to 1079 μg/kg. Contamination levels were above the established maximum limit for unprocessed cereals, other than maize, in as many as seventeen samples. These results were compared to the results of investigation of deoxynivalenol and fumonisin content, established in our previous work on the same samples. The results obtained were also compared to those of the neighboring countries where the relevant data existed and to the data of previous studies in our country.Na prisustvo zearalenona analizirano je ukupno 45 uzoraka pšenice sa tri različite lokacije u Vojvodini. Korišćene su analitičke metode zasnovane na prečišćavanju ekstrakcijom na čvrstoj fazi, te kvantifikacija tečnom hromatografijom, nakon validacije metode. Granica detekcije za zearalenon u pšenici je iznosila 18,6 μg/kg, a granica određivanja 56,5 μg/kg. Efikasnost metode je bila u opsegu od 86% do 97%. Zearalenon je bio prisutan u 53,3% ispitivanih uzoraka, sa prosečnim sadržajem od 330 μg/kg. Dobijene vrednosti sadržaja zearalenona su bile u opsegu od 68 μg/kg do 1079 μg/kg. U čak sedamnaest uzoraka je pronađena koncentracija ovog toksina koja prevazilazi maksimalni dozvoljeni sadržaj zearalenona u netretiranim žitaricama. Ovi rezultati su upoređeni sa vrednostima sadržaja deoksinivalenola i fumonizina u istim uzorcima dobijenim u našim prethodnim istraživanjima. Rezultati su takođe upoređeni sa dostupnim rezultatima dobijenim u našoj i susednim zemljama tokom prethodnih godina