Characterization of the Hsp100 disaggregase from sugarcane (SHsp101) for chaperone like activity in a yeast system

FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULOThe Hsp104/ClpB protein subfamily is unique in its ability to reactivate protein aggregates, which are implicated in several human and animal diseases. However, when compared to the unicellular yeast Hsp104 and bacterial ClpB disaggregases, the multicellu26478487FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULOFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO2012/50161-8We thank Claudio Masuda (UFRJ) for gifting the BY4741 yeast strain and isogenic Hsp104 knockout, and Doug Cyr (University of North Carolina) for the Rnq-mRFP yeast expression plasmid, and the W303 [RNQ+] yeast strai