Metabolic screening for PKS and NRPS in endophytic actinobacteria from citrus reticulata
- Cruz, Pedro L. R. da
- Giarola, Leila R.
- Moraes, Suellen da Silva
- Silva, Déborah Ellen S. G. da
- Marcon, Joelma
- Azevedo, João L.
- Araújo, Welington L.
- Oliveira, Luciana G. de
DOIAbstract
Polyketides and non-ribosomal peptides are natural products widely found in bacteria, fungi and plants. The biological activities associated with these metabolites have attracted special attention in biopharmaceutical studies. Polyketide synthases act similarly to fatty acids synthetases and the whole multi-enzymatic set coordinating precursor and extending unit selection and reduction levels during chain growth. Acting in a similarly orchestrated model, non-ribosomal peptide synthetases biosynthesize NRPs. PKSs-I and NRPSs enzymatic modules and domains are collinearly organized with the parent gene sequence. This arrangement allows the use of degenerated PCR primers to amplify targeted regions in the genes corresponding to specific enzymat...
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