Insights into population structure of East African sweetpotato cultivars from hybrid assembly of chloroplast genomes.
- Zhou, C
- Duarte, T
- Silvestre, R
- Rossel, G
- Mwanga, ROM
- Khan, A
- George, AW
- Fei, Z
- Yencho, GC
- Ellis, D
- Coin, LJM
DOIAbstract
Background: The chloroplast (cp) genome is an important resource for studying plant diversity and phylogeny. Assembly of the cp genomes from next-generation sequencing data is complicated by the presence of two large inverted repeats contained in the cp DNA. Methods: We constructed a complete circular cp genome assembly for the hexaploid sweetpotato using extremely low coverage (<1×) Oxford Nanopore whole-genome sequencing (WGS) data coupled with Illumina sequencing data for polishing. Results: The sweetpotato cp genome of 161,274 bp contains 152 genes, of which there are 96 protein coding genes, 8 rRNA genes and 48 tRNA genes. Using the cp genome assembly as a reference, we constructed complete cp genome assemblies for a further 17 sweetpo...
Add to ORKG