Glycosylated amyloid-like proteins in the structural extracellular polymers of aerobic granular sludge enriched with ammonium-oxidizing bacteria

A new type of structural extracellular polymers () was extracted from aerobic granular sludge dominated by ammonium-oxidizing bacteria. It was analyzed by Raman and spectroscopy to characterize specific amino acids and protein secondary structure, and by - with different stains to identify different glycoconjugates. Its intrinsic fluorescence was captured to visualize the location of the extracted in the nitrifying granules, and its hydrogel-forming property was studied by rheometry. The extracted is abundant with cross ß-sheet secondary structure, contains glycosylated proteins/polypeptides, and rich in tryptophan. It forms hydrogel with high mechanical strength. The extraction and discovery of glycosylated proteins and/or amyloids further shows that conventionally used extraction and characterization techniques are not adequate for the study of structural extracellular polymers in biofilms and/or granular sludge. Confirming amyloids secondary structure in such a complex sample is challengeable due to the possibility of amyloids glycosylation and self-assembly. A new definition of extracellular polymers components which includes glycosylated proteins and a better approach to studying them is required to stimulate biofilm research