Realistic simulation and experiment reveals the importance of scatterer microstructure in optical coherence tomography image formation

Realistic simulation of image formation in optical coherence tomography, based on Maxwell’s equations, has recently been demonstrated for sample volumes of practical significance. Yet, there remains a limitation whereby reducing the size of cells used to construct a computational grid, thus allowing for more realistic representation of scatterer microstructure, necessarily reduces the overall sample size that can be modelled. This is a significant problem since, as is well known, the microstructure of a scatterer significantly influences its scattering properties. Here we demonstrate that optimized scatterer design can overcome this problem resulting in good agreement between simulated and experimental images for a structured phantom. This approach to OCT image simulation allows for image formation for biological tissues to be simulated with unprecedented realism