Direct electron transfer of glucose oxidase immobilized in an ionic liquid reconstituted cellulose-carbon nanotube matrix

Conductive cellulose-multiwalled carbonnanotube (MWCNT) matrix with a porous structure and good biocompatibility has been prepared using a room temperature ionicliquid (1-ethyl-3-methylimidazolium acetate) as solvent. Glucoseoxidase (GOx) was encapsulated in this matrix and thereby immobilized on a glassy carbon surface. The directelectrontransfer and electrocatalysis of the encapsulated GOx has been investigated using cyclic voltammetry and chronoamperometry. The GOx exhibited a pair of stable, well defined and nearly symmetric reversible redox peaks. The experimental results also demonstrate that the immobilized GOx retains its biocatalytic activity toward the oxidation of glucose and therefore can be employed in a glucose biosensor. The results show that the bioelectrode modified by the cellulose-MWCNT matrix has potential for use in biosensors and other bioelectronics devices