慢性歯周炎特有の歯肉溝滲出液中エクソソームにおけるmiRNAの検出

MicroRNAs suppress the expression of target genes and extensively regulate the expression of genes involved in growth and disease. The purpose of this study was to detect exosomes from gingival crevicular fluid (GCF), and to elucidate disease-specific exosomal miRNA expression in GCF from patients with chronic periodontal disease. Comprehensive microRNA expression analysis was performed using exosomal miRNA in GCF to compare the GCF of patients with chronic periodontitis disease with that of healthy participants. The candidate exosomal miRNAs were analyzed using real-time quantitative polymerase chain reaction (qPCR). The presence of exosomes was observed by measuring the particle size, as well as by detecting CD9/CD63 and observing transmission electron microscope images (TEM). Eleven exosomal miRNAs were identified as differentially expressed more than 8 times lower than in healthy participants by global analysis. Of the miRNA, qPCR analysis revealed that the expression of hsa-mir-204-3p was lower in GCF collected from affected and unaffected sites of patients with chronic periodontal disease, compared with those collected from healthy individuals. This suggests that hsa-mir-204-3p might be associated with periodontal pathology.大阪歯科大学2020年