Marine invertebrate cell culture

Kulture životinjskih stanica su značajan alat u staničnoj i molekularnoj biologiji budući pružaju izvrsne modele sustava za proučavanje fiziologije i biokemije stanica, procesa mutageneze i karcinogeneze te učinka lijekova i toksičnih spojeva na stanice. U ovom radu je dan pregled dosadašnjih saznanja na području staničnih kultura morskih beskralježnjaka. Kulture matičnih i embrionalnih stanica ovih organizama predstavljaju novi eksperimentalni model koji karakteriziraju visoka razina fizioloških i sintetskih procesa. Primarne kulture morskih beskralježnjaka koje su dosada uspješno kultivirane su: epitelne stanice spužvi, rakova, bodljikaša, plaštenjaka i svitkoglavaca; neuroni žarnjaka, rakova i mekušaca; krvne stanice rakova, mekušaca i plaštenjaka te embrionalne stanice spužvi, žarnjaka i bodljikaša. Nažalost, još nije uspostavljena stanična linija nekog morskog beskralježnjaka, što ukazuje na nedostatak informacija o potrebama njihovih stanica (pH, nutrijenti, temperatura, supstrat) u in vitro uvjetima.Animal cell cultures are important tool in cell and molecular biology that provides an excellent model systems for studying cell physiology and biochemistry, the processes of mutagenesis and carcinogenesis, as well as the effects of drugs and toxic compounds on cells. This paper provides an overview of current knowledge in the field of marine invertebrate cell cultures. The stem and embryonic cell cultures of these organisms represent new experimental model characterized by a high variety of physiological and synthetic processes. The primary cultures of marine invertebrates that have been successfully cultivated are: epithelial cells of spongia, crustacea, echinodermata, tunicata and cephalochordata; neurons of cnidaria, crustacea and mollusca; blood cells of crustacea, mollusca and tunicata, and embryonic cells of spongia, cnidaria and echinodermata. Unfortunately, a cell line of a marine invertebrate has not yet been established, indicating the lack of information about their cells requirements (pH, nutrients, temperature, substrate) when grown in vitro