MicroRNAs (miRNAs) are critical to somatic cell reprogramming into induced pluripotent stem cells (iPSCs), however, exactly how miRNA expression changes support the transition to pluripotency requires further investigation. Here we use a murine secondary reprogramming system to sample cellular trajectories towards iPSCs or a novel pluripotent ‘F-class’ state and perform small RNA sequencing. We detect sweeping changes in an early and a late wave, revealing that distinct miRNA milieus characterize alternate states of pluripotency. miRNA isoform expression is common but surprisingly varies little between cell states. Referencing other omic data sets generated in parallel, we find that miRNA expression is changed through transcriptional and po...
Human pluripotent stem cells (hPSCs) represent a promising platform for studying embryonic developme...
Early mammalian development is driven by precise molecular changes that enable cells to establish, m...
Somatic cells are reprogrammed with reprogramming factors to generate induced pluripotent stem cells...
MicroRNAs (miRNAs) are critical to somatic cell reprogramming into induced pluripotent stem cells (i...
BackgroundMiRNAs often operate in feedback loops with transcription factors and represent a key mech...
Supplemental material: http://rnajournal.cshlp.org/content/suppl/2011/12/01/rna.028878.111.DC1.htmlO...
In addition to the well-characterized Induced Pluripotent Stem cells (iPSCs) that closely resemble E...
Noncoding RNAs have emerged as important determinants of pluripotency and reprogramming. In this iss...
Ectopic expression of specific factors such as Oct4, Sox2, and Klf4 (OSK) is sufficient to reprogram...
SummaryEctopic expression of specific factors such as Oct4, Sox2, and Klf4 (OSK) is sufficient to re...
AbstractPluripotent stem cells display a unique expression pattern of microRNAs (miRNAs). These ~22 ...
SummaryReprogramming methodologies have provided multiple routes for achieving pluripotency. However...
© 2020 National Academy of Sciences. All rights reserved. Pluripotent embryonic stem cells (ESCs) co...
MicroRNAs (miRNAs) are a class of endogenous non-coding RNAs that post-transcriptionally co‐regulate...
Induced pluripotent stem (iPS) cells were first generated by forced expression of transcription fact...
Human pluripotent stem cells (hPSCs) represent a promising platform for studying embryonic developme...
Early mammalian development is driven by precise molecular changes that enable cells to establish, m...
Somatic cells are reprogrammed with reprogramming factors to generate induced pluripotent stem cells...
MicroRNAs (miRNAs) are critical to somatic cell reprogramming into induced pluripotent stem cells (i...
BackgroundMiRNAs often operate in feedback loops with transcription factors and represent a key mech...
Supplemental material: http://rnajournal.cshlp.org/content/suppl/2011/12/01/rna.028878.111.DC1.htmlO...
In addition to the well-characterized Induced Pluripotent Stem cells (iPSCs) that closely resemble E...
Noncoding RNAs have emerged as important determinants of pluripotency and reprogramming. In this iss...
Ectopic expression of specific factors such as Oct4, Sox2, and Klf4 (OSK) is sufficient to reprogram...
SummaryEctopic expression of specific factors such as Oct4, Sox2, and Klf4 (OSK) is sufficient to re...
AbstractPluripotent stem cells display a unique expression pattern of microRNAs (miRNAs). These ~22 ...
SummaryReprogramming methodologies have provided multiple routes for achieving pluripotency. However...
© 2020 National Academy of Sciences. All rights reserved. Pluripotent embryonic stem cells (ESCs) co...
MicroRNAs (miRNAs) are a class of endogenous non-coding RNAs that post-transcriptionally co‐regulate...
Induced pluripotent stem (iPS) cells were first generated by forced expression of transcription fact...
Human pluripotent stem cells (hPSCs) represent a promising platform for studying embryonic developme...
Early mammalian development is driven by precise molecular changes that enable cells to establish, m...
Somatic cells are reprogrammed with reprogramming factors to generate induced pluripotent stem cells...