Two-photon imaging
- Ricard, C.
- Coles, J. A.
- Serduc, R.
- van der Sanden, B.
- Verant, P.
- Vial, J.-C.
DOIAbstract
A two-photon microscope is basically a scanning confocal microscope that uses for excitation near-infrared light delivered in intense, femtosecond pulses. It allows imaging with the spatial resolution of a conventional optical microscope (<1 μm) but can perform such imaging through several hundred micrometers of brain tissue. In this article, the physical principles of two-photon imaging are explained, and imaging in the cortex of the living mouse of neurons, glia, and microvasculature is described. Finally, the advantages of two-photon microscopy for studying neuropathologies including tumors are summarized
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