Gating in plant plasma membrane aquaporins: the involvement of leucine in the formation of a pore constriction in the closed state
- Canessa Fortuna, Agustina
- Zerbetto de Palma, Gerardo Gabriel
- Aliperti Car, Lucio
- Armentia, Luciano
- Vitali, Victoria Andrea
- Zeida, Ari
- Estrin, Dario Ariel
- Alleva, Karina Edith
DOIAbstract
The control of water permeability in plant PIP2 aquaporins has become a paradigmatic case study of the capping mechanism for pore closure in water channels. From structural data, it has been postulated that the gating process in PIP2 involves a conformational rearrangement in cytosolic loopD that generates an obstruction to the transport of water molecules inside the aquaporin pore. BvPIP2;2 is a PIP2 aquaporin from Beta vulgaris whose pH response has been thoroughly characterized. In this work, we study the participation of Leu206 in BvPIP2;2 gating triggered by cytosolic acidification and show that this residue acts as a plug that blocks water transport. Based on data obtained from in silico and in vitro studies, we demonstrate that Leu20...
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